• Publications

    In reverse chronological order

    Papers:

    (*: Corresponding author)

    1. Peng Zhang#, Jun Lin#, Yuan Lu*. Advances in cell-free protein synthesis. Chinese Journal of Bioprocess Engineering, 2018, 16(01): 59-66
    2. Zhidan Liu, Buchun Si, Jiaming Li, Jianwei He, Chong Zhang, Yuan Lu, Yuanhui Zhang, Xin-Hui Xing. Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up. Current Opinion in Biotechnology, 2018, 50: 25-31
    3. Yan Wu, Yaqiao Hao, Xuan Wei, Qi Shen, Xuanwei Ding, Liyan Wang, Hongxin Zhao*, Yuan Lu*. Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes. Biotechnology for Biofuels, 2017, 10: 248
    4. Dong Liu, Yunze Sun, Yuhao Li, Yuan Lu*. Perturbation of formate pathway and NADH pathway acting on the biohydrogen production. Scientific Reports, 2017, 7:9587
    5. Yishu Yan, Changge Guan, Shanshan Du, Wenming Zhu, Yang Ji, Nan Su, Xiang Mei, Dong He, Yuan Lu, Chong Zhang, Xin-Hui Xing. Effects of Enzymatically Depolymerized Low Molecular Weight Heparins on CCl4-Induced Liver Fibrosis. Frontiers in Pharmacology, 2017, 8:514
    6. Yishu Yan, Shanshan Du, Yang Ji, Nan Su, Yi Wang, Dong He, Yuan Lu, Chong Zhang, Xin-Hui Xing. Discovery of enzymatically depolymerized heparins capable of treating Bleomycin-induced pulmonary injury and fibrosis in mice. Carbohydrate Polymers, 2017,174: 82-88
    7. Yuan Lu*. Cell-free synthetic biology: Engineering in an open world. Synthetic and Systems Biotechnology, 2017, 2 (1):23-27 
    8. Yishu Yan, Yang Ji, Nan Su, Xiang Mei, Yi Wang, Shanshan Du, Wenming Zhu, Chong Zhang, Yuan Lu, Xin-Hui Xing. Non-anticoagulant effects of low molecular weight heparins in inflammatory disorders: A review. Carbohydrate Polymers, 2017, 160: 71-81
    9. Shouchi Zhang, Qiheng Lai, Yuan Lu, Zhidan Liu, Tianmin Wang, Chong Zhang, Xin-Hui Xing. Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria. Journal of Bioscience and Bioengineering, 2016, 122 (4): 482-487
    10. Yuan Lu, James R. Swartz. Functional properties of flagellin as a stimulator of innate immunity. Scientific Reports, 2016, 6:18379
    11. Yuan Lu, Hongxin Zhao, Chong Zhang, Xin-Hui Xing. Insights into the global regulation of anaerobic metabolism for improved biohydrogen production. Bioresource Technology, 2016, 200: 35-41
    12. Yuan Lu, Wei Chan, Benjamin Y. Ko, Christopher C. VanLang, James R. Swartz. Assessing sequence plasticity of a virus-like nanoparticle by evolution toward a versatile scaffold for vaccines and drug delivery. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 2015, 112(40): 12360-12365 (Direct submission)    News & Features: Stanford News, The Stanford Daily, ScienceDaily, Phys.org, Reddit, FierceBiotech Research, World Pharma News, SciCentral, Varsity, GlobalNews Connect, NewsReality, etc.
    13. Hongxin Zhao#, Yuan Lu#, Liyan Wang, Chong Zhang, Cheng Yang, Xin-Hui Xing. Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes. Bioresource Technology, 2015, 194: 99-107 (#Co-first author)
    14. Yuan Lu, John P. Welsh, James R. Swartz. Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 2014, 111(1): 125-130 (Direct submission)    News & Features: Stanford News, The Stanford Daily, Mountain View Voice, Palo Alto Online, ScienceDaily, RT Magazine, WSRQ Sarasota 1220AM & 106.9 FM, GEN News, HighBeam Research, Scope Blog, Futurity, Daily World 24, HealthCanal, Inform Special, Vaccine News Daily, etc.
    15. Yuan Lu, John P. Welsh, Wei Chan, James R. Swartz. Escherichia coli-based cell free production of flagellin and ordered flagellin display on virus-like particles. Biotechnology and Bioengineering, 2013, 110(8): 2073-2085 (Spotlight article)
    16. Zhidan Liu, Chong Zhang, Yuan Lu, Xiao Wu, Lang Wang, Linjun Wang, Bing Han, Xin-Hui Xing. States and challenges for high-value biohythane production from waste biomass by dark fermentation technology. Bioresource Technology, 2013, 135: 292–303
    17. Yuan Lu, Chong Zhang, Hongxin Zhao, Xin-Hui Xing. Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum. Applied Biochemistry and Biotechnology, 2012, 167(4): 732-741
    18. Yuan Lu, Qiheng Lai, Chong Zhang, Hongxin Zhao, Xin-Hui Xing. Alteration of energy metabolism in Enterobacter aerogenes by external addition of pyrophosphates and overexpression of polyphosphate kinase for enhanced hydrogen production. Journal of Chemical Technology & Biotechnology, 2012, 87(7): 996-1003
    19. John P. Welsh, Yuan Lu, Xiao-Song He, Harry B. Greenberg, James R. Swartz. Cell-free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens. Biotechnology and Bioengineering, 2012, 109(12): 2962–2969
    20. Hao Zheng, Chong Zhang, Yuan Lu, Pei-Xia Jiang, Xin-Hui Xing. Alteration of anaerobic metabolism in Escherichia coli for enhanced hydrogen production by heterologous expression of hydrogenase genes originating from Synechocystis sp. Biochemical Engineering Journal, 2012, 37(21):15875–15885
    21. Kun Ma, Hongxin Zhao, Chong Zhang, Yuan Lu, Xin-Hui Xing. Impairment of NADH dehydrogenase for increased hydrogen production and its effect on metabolic flux redistribution in wild strain and mutants of Enterobacter aerogenes. International Journal of Hydrogen Energy, 2012, 37(21):15875–15885
    22. He-Ping Li, Zhi-Bin Wang, Nan Ge, Pei-Si Le, Hao Wu, Yuan Lu, Li-Yan Wang, Chong Zhang, Cheng-Yu Bao, Xin-Hui Xing. Studies on the physical characteristics of the radio-frequency atmospheric-pressure glow discharge plasmas for the genome mutation of Methylosinus trichosporium. IEEE Transactions on Plasma Science, 2012, 40(11): 2853-2860
    23. Yuan Lu, Chong Zhang, Qiheng Lai, Hongxin Zhao, Xin-Hui Xing. Improved hydrogen production under microaerophilic conditions by overexpression of polyphosphate kinase in Enterobacter aerogenes. Enzyme and Microbial Technology, 2011, 48:187-192
    24. Yuan Lu, Liyan Wang, Kun Ma, Guo Li, Chong Zhang, Hongxin Zhao, Qiheng Lai, He-Ping Li, Xin-Hui Xing. Characteristics of hydrogen production of an Enterobacter aerogenes mutant generated by a new atmospheric and room temperature plasma (ARTP). Biochemical Engineering Journal, 2011,55:17-22
    25. Yuan Lu, Hongxin Zhao, Chong Zhang, Qiheng Lai, Xi Wu, Xin-Hui Xing. Alteration of hydrogen metabolism of ldh-deleted Enterobacter aerogenes by overexpression of NAD(+)-dependent formate dehydrogenase. Applied Microbiology and Biotechnology, 2010, 86:255-262
    26. Yuan Lu, Liyan Wang, Yuan Xue, Chong Zhang, Xin-Hui Xing, Kai Lou, Zhidong Zhang, Yong Li, Guifeng Zhang, Jingxiu Bi, Zhiguo Su. Production of violet pigments by a newly isolated psychrotrophic bacterium from the glacier in Xinjiang of China. Biochemical Engineering Journal, 2009,43(2):135-141
    27. Yuan Lu, Qiheng Lai, Chong Zhang, Hongxin Zhao, Kun Ma, Xuebing Zhao, Hongzhang Chen, Dehua Liu, Xin-Hui Xing. Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process. Bioresource Technology, 2009,100(12):2889-2895
    28. Yuan Lu, Hongxin Zhao, Chong Zhang, Qiheng Lai, Xin-Hui Xing. Perturbation of formate pathway for hydrogen production by expressions of formate hydrogen lyase and its transcriptional activator in wild Enterobacter aerogenes and its mutants. International Journal of Hydrogen Energy, 2009, 34:5072-5079
    29. Yuan Lu, Hongxin Zhao, Chong Zhang, Qiheng Lai, Xi Wu, Xin-Hui Xing. Expression of NAD(+)-dependent formate dehydrogenase in Enterobacter aerogenes IAM1183 and its modification for the anaerobic metabolism and hydrogen production. Biotechnology Letters, 2009, 31:1525-1530
    30. Hongxin Zhao, Kun Ma, Yuan Lu, Chong Zhang, Xin-Hui Xing. Cloning and knockout of formate hydrogen lyase and H2-uptake hydrogenase genes in Enterobacter aerogenes for enhanced hydrogen production. International Journal of Hydrogen Energy, 2009, 34: 186-194
    31. Haisheng Wang, Peixia Jiang, Yuan Lu, Zhiyong Ruan, Ruibo Jiang, Xin-Hui Xing, Kai Lou, Dong Wei. Optimization of culture conditions for violacein production by a new strain of Duganella sp. B2. Biochemical Engineering Journal, 2009, 44(2-3):119-124
    32. Yuan Lu, Chong Zhang, Hongxin Zhao, Kun Ma, Qiheng Lai, Xin-hui Xing. Advances in Production Technology of Biological Fuel Gas. Chemical Industry (Chinese). 2008,26(6): 31-38
    33. Haisheng Wang, Yuan Lu, Yuan Xue, Zhiyong Ruan, Ruibo Jiang, Xin-Hui Xing, Kai Lou. Separation, purification and structure identification of purple pigment s from Duganella B2. Journal of Chemical Industry and Engineering (Chinese), 2008,59(3): 630-635
    34. Hongxin Zhao, Cheng Yang, Yuan Lu, Rong Shi, Qiheng Lai, Xin-Hui Xing. Characteristic of enzymatic phosphorylation of inosine by Enterobacter aerogenes. Journal of Tsinghua University (Science and Technology), 2008, 48(12): 2122-2125
    35. Haisheng Wang, Xiaoxia Zhang, Yuan Lu, Zhiyong Ruan, Xin-Hui Xing, Ruibo Jiang. Recent research progress of bacterial violacein. Chemical Industry and Engineering Progress (China). 2008, 27(3): 315-321
    36. Yuan Lu, Chong Zhang, Xin-Hui Xing. Effect of cultivation conditions on hydrogen production by Clostridium paraputrificum M-21. Chinese Journal of Bioprocess Engineering, 2004,2(2): 41-45

    Patents:

    1. Yuan Lu, James Swartz. Disulfide Stabilized Foldon Polypeptide. WO2013177283.
    2. Yuan Lu, James Swartz. Ordered Flagellin Array as an Immunostimulant. WO2013177440.
    3. Yuan Lu, James Swartz. Influenza Vaccine Constructs. WO2013177444.
    4. Yuan Lu, James Swartz. Stabilized Hepatitis B Core Polypeptide. WO2014116730.
    5. Cem Albayrak, James Swartz, Yuan Lu. Synthesis of Linear and Branched Polymers of Polypeptides Through Direct Conjugation. US 9255255 B2
    6. Xin-Hui Xing, Yuan Lu, Chong Zhang, Hongxin Zhao, Kun Ma, Qiheng Lai. A degradation method for the lignocellulosic biomasses (CN101608194 B).
    7. Xin-Hui Xing, Yuan Lu, Chong Zhang, Qiheng Lai, Xi Wu. An genetically engineered strain for the hydrogen production and its application (CN101613671 B).
    8. Xin-Hui Xing, Yuan Lu, Chong Zhang, Qiheng Lai. A method for the enhanced bacterial hydrogen fermentation production (CN101597624 B).
    9. Xin-Hui Xing, Yuan Lu, Chong Zhang, Qiheng Lai. An improved method for the biohydrogen production and the superior strain (CN101597587 B).
    10. Xin-Hui Xing, Yuan Lu, Chong Zhang, Hongxin Zhao, Kun Ma, Qiheng Lai. A method for the hydrogen or methane production (CN101289672 A).
    11. Xin-Hui Xing, Yuan Lu, Hongxin Zhao, Chong Zhang, Kun Ma, Qiheng Lai. A method for the hydrogen production (CN101294167 B).
    12. Xin-Hui Xing, Hongxin Zhao, Yuan Lu, Chong Zhang, Kun Ma, Peixia Jiang, Tao Su, Cheng Yang, Liyan Wang. A protein related with the hydrogen production and its application (CN101633691 B).
    13. Xin-Hui Xing, Haisheng Wang, Yuan Lu, Yuan Xue, Ruibo Jiang, Kai Lou, Dong Wei. A strain of Duganella sp. and its application (CN101139564 B).
    14. Xin-Hui Xing, Kai Lou, Yuan Lu, Dong Wei, Jun Mao, Zhidong Zhang. A method for the violet pigment production and special strains (CN100335613 C).
    15. Xin-Hui Xing, Hongxin Zhao, Kun Ma, Yuan Lu, Chong Zhang, Liyan Wang, Cheng Yang, Qiheng Lai. A protein related with the hydrogen production and its application (CN101633690 B).
    16. Xin-Hui Xing, Hongxin Zhao, Kun Ma, Yuan Lu, Liyan Wang, Chong Zhang. A genetic engineering strain for the hydrogen production and its application (CN101608171 A).
    17. Xin-Hui Xing, Hongxin Zhao, Kun Ma, Yuan Lu, Liyan Wang, Chong Zhang, Rong Shi. A genetic engineering strain for the hydrogen production and its application (CN101608170 B).
    18. Hongxin Zhao, Xin-Hui Xing, Cheng Yang, Yuan Lu, Chong Zhang, Liyan Wang. A biological synthetic method for the production of inosine (CN100552037 C).

    Invited talks and presentations:

    1. Yuan Lu. Cell-free synthetic biotechnology: an emerging engineering strategy to revolutionize the health industry. 3rd International Symposium of Food Science and Human Wellness. August 17, 2017, Changchun, Jilin province, China.
    2. Yuan Lu. Cell-free synthetic biology and biomedical macromolecular engineering. June 6, 2017, Guangxi University, Guangxi, China.
    3. Yuan Lu. Cell-free synthetic biology: An emerging strategy to revolutionize the biomedical industry. May 2, 2017, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning province, China.
    4. Yuan Lu. Cell-free synthetic biology: An emerging strategy to revolutionize the biomedical industry. April 14, 2017, Tsinghua University-The University of Tokyo Joint Workshop for Fontiers in Bioengineering and Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
    5. Yuan Lu. Cell-free synthetic biology: an emerging engineering strategy to revolutionize the biopharmaceutical industry. March 22, 2017, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
    6. Yuan Lu. Cell-free synthetic biology: an emerging engineering strategy to revolutionize the biopharmaceutical industry. March 21, 2017, Tianjin University, Tianjin, China.
    7. Yuan Lu. Cell-free synthetic biology: an advanced engineering strategy to revolutionize the biopharmaceutical industry. Tokyo Tech - Tsinghua University Joint Forum, January 20, 2017, Tokyo Institute of Technology, Suzukakedai Campus, Yokohama, Japan.
    8. Yuan Lu. Cell-free biosynthesis: an advanced engineering strategy to revolutionize the biopharmaceutical industry. Silk Road Young Scholars Forum, December 22, 2016, Xi’an Jiaotong University, Xi’an, Shaanxi, China.
    9. Yuan Lu. Cell-free synthetic biology: a bottom-up strategy to revolutionize the biomedical industry. The 3rd International Conference on Applied Biotechnology, November 27, 2016, Tianjin University of Science and Technology, Tianjin, China.
    10. Yuan Lu. Bottom-up virus engineering using cell-free synthetic biology. May 12, 2016, Tsinghua University, Beijing, China.
    11. Yuan Lu. Cell-free biotechnology and biomedical macromolecular engineering. April 29, 2016, Tianjin University, Tianjin, China.
    12. Yuan Lu. Cell-free biotechnology and macromolecular engineering for biomedical applications. April 13, 2016, Peking University, Beijing, China.
    13. Yuan Lu. Cell-free biotechnology and macromolecular engineering for biomedical applications. March 11, 2016, Tsinghua University, Beijing, China.
    14. Yuan Lu. Virus engineering using cell-free synthetic biology. February 23, 2016, The City University of Hong Kong, Hong Kong, China.
    15. Yuan Lu. Virus engineering using cell-free synthetic biology. January 26, 2016, The Hong Kong Polytechnic University, Hong Kong, China.
    16. Yuan Lu. Synthetic biology: an integration strategy to revolutionize the life sciences industry. January 7, 2016, National University of Singapore, Singapore.
    17. Yuan Lu. A golden age for cell-free synthetic biology: its application in pharmaceutical research. September 29, 2015, Kashiwa campus, The University of Tokyo, Japan.
    18. Yuan Lu. Application of cell-free synthetic biology in the synthesis of highly stable virus-like particles. Seminar on Green Sustainable Chemistry in Tottori (2014-3). December 5, 2014, Tottori University, Japan.
    19. Yuan Lu. Golden age for cell-free synthetic biology: its application in medical fields. Frontiers in Industrial Biocatalysis — Tsinghua Forum. May 10, 2014, Tsinghua University, Beijing, China.

    International conference presentations:

    1. Yuan Lu. Cell-free synthetic biology: a bottom-up strategy to revolutionize the biomedical industry. I-BIOP 2016, December 17, 2016, Busan, Korea.
    2. Yuan Lu. Cell-free synthetic biology: a bottom-up metabolic engineering strategy to revolutionize the biomedical industry. International Conference on Metabolic Science (ICMS 2016), October 20-23, 2016, Shanghai, China.
    3. Yuan Lu, Kosuke Minamihata, and Teruyuki Nagamune. Buildup of a versatile virus-like particle (VLP) platform by genetically encoded SpyTag-SpyCatcher chemistry. TSBMI-9, February 12, 2016, Tokyo, Japan.
    4. Yuan Lu, Kosuke Minamihata, and Teruyuki Nagamune. Synthesis of protein-based biomaterials by genetically encoded SpyTag-SpyCatcher chemistry. PACIFICHEM 2015, December 15-20, 2015, Honolulu, Hawaii, USA.
    5. Yuan Lu. A golden age for cell-free synthetic biology: its application in pharmaceutical research. International Forum on Green Integrative Biotechnology (IF-GIB), October 22, 2014, Beijing, China.
    6. Yuan Lu, James Swartz. Production of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines. AIChE Annual Meeting, November 3-8, 2013, San Francisco, CA, USA.
    7. Yuan Lu, James Swartz. Cell free production of flagellin as a vaccine adjuvant and its ordered display on virus-like particles. AIChE Annual Meeting, November 3-8, 2013, San Francisco, CA, USA.
    8. Yuan Lu, James Swartz. Synthesis of highly stable self-assembled virus-like particles. AIChE Annual Meeting, November 3-8, 2013, San Francisco, CA, USA.
    9. Christopher C. VanLang, Wei Chan, Yuan Lu, James Swartz. Rational Design of Polypeptides for the Functionalization of Novel Nanoparticles. AIChE Annual Meeting, November 3-8, 2013, San Francisco, CA, USA.
    10. James Swartz, Yuan Lu, John Welsh, Wei Chan. Multiply activated VLP influenza vaccines. Vaccine Technology IV, May 20-25, 2012, Albufeira, Portugal.
    11. Yuan Lu, John Welsh, James Swartz. Broadly protective influenza vaccines based on the conserved hemagglutinin stalk region. AIChE Annual Meeting, October 16-21, 2011, Minneapolis, MN, USA.
    12. Xin-Hui Xing, Yuan Lu, Chong Zhang, Hongxin Zhao and Qiheng Lai. Perturbation of hydrogen metabolism by introduction of intracellular NADH regeneration in typical bacteria. Fifth Sino-US Conference for Chemical Engineering. October 13-16, 2009, Beijing, China.
    13. Yuan Lu, Hongxin Zhao, Chong Zhang, Kun Ma, Qiheng Lai, Xin-Hui XING. Effects of phosphate and overexpression of polyphosphate kinase on hydrogen production and metabolic flux distribution in Enterobacter aerogenes. 10th Korea-Japan-China Joint Symposium on Enzyme Engineering, November 2-5, 2008, Busan, Korea.
    14. Yuan Lu, Chong Zhang, Hongxin Zhao, Kun Ma, Qiheng Lai, Xuebing Zhao, Hongzhang Chen, Dehua Liu, Xin-Hui Xing. Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process. 13th International Biotechnology Symposium, October 12-17, 2008, Dalian, China.
    15. Yuan Lu, Xin-Hui Xing. Hydrogen and Methane Production from Biomass by a Two-Stage Fermentation Process. International Symposium on Advanced Biological Engineering and Science, May 19, 2008, Tsinghua University, Beijing, China.
    16. Yuan Lu, Chong Zhang, Hongxin Zhao, Kun Ma, Xuebing Zhao, Hongzhang Chen, Dehua Liu, Xin-Hui Xing. Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process. The 2nd International Symposium on Advanced Biological Engineering and Science(ISABES-2), March 31-April 1, 2008, Beijing, China.
    17. Yuan Lu, Xin-Hui Xing, Hongzhang Chen. Hydrogen and methane production from biomass by a two-stage fermentation process. International Symposium on Clean Energy Technology 2007 (ISCET 2007) in conjunction with The 3rd International Symposium on Bioenergy and Bioprocess Engineering (ISBBE 2007), November 21-23, 2007, Shanghai, China. (Best Poster Award)
    18. Hongxin Zhao, Kun Ma, Yuan Lu, Chong Zhang, Brian Caliando, Xinhui Xing. Functional analysis of FHL cluster of Enterobacter aerogenes and genetic knockout for enhanced hydrogen production. The 2007 Asian Bio-Hydrogen Symposium/Asia Bio-HyLinks Meeting, November 9-11, 2007, Daejeon, Korea, p248.
    19. Hongxin Zhao, Qiheng Lai, Cheng Yang, Yuan Lu, Xinhui Xing. Characteristics of MBP-PhoC fusion protein for enzymatic phosphorylation in 5’-position of inosine. The First International Symposium on Sustainable Chemical Product and Process Engineering (SCPPE2007), September 25-28, 2007, Guangzhou, China.
    20. Yuan Lu, Xin-Hui Xing, Hongzhang Chen. Hydrogen and methane production from biomass by a two-stage fermentation process. The First International Symposium on Sustainable Chemical Product and Process Engineering (SCPPE2007), September 25-28, 2007, Guangzhou, China.
    21. Yuan Lu, Kai Lou, Zhi-Dong Zhang, Xin-Hui Xing. Production of blue pigment by a newly separated psychrotrophic bacterium of Pseudomonas fluorescens B1. The 8th China-Japan-Korea Joint Symposium on Enzyme Engineering, October 24-28, 2004, Hangzhou, China. 
    22. Yuan Lu, Kai Lou, Zhi-Dong Zhang and Xin-Hui Xing. Production of blue pigment by a newly separated psychrotrophic bacterium of Pseudomonas fluorescens B1. Tsinghua-Yokohama Mini Academic Intercommunion on Bioengineering, 2004, Tsinghua University, Beijing, China.